Mouse Ccd1/DIXDC1 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF5599X Datasheet / COA / SDS

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AF5599X-100UG

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All Ccd1/DIXDC1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Ccd1/DIXDC1 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Ccd1/DIXDC1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Ccd1/DIXDC1
Gln481-Asn711
Accession # Q80Y83

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ccd1/DIXDC1

Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.

Long Name

Coiled-coil DIX1/DIX Domain Containing 1

Entrez Gene IDs

85458 (Human); 330938 (Mouse); 363062 (Rat)

Alternate Names

Ccd1; Coiled-coil protein DIX1; Coiled-coil-DIX1; DIX domain containing 1; DIX domain-containing protein 1; Dixdc1; Dixin; Kiaa1735; KIAA1735coiled-coil-DIX1