Mouse Ccd1/DIXDC1 Antibody

Catalog # Availability Size / Price Qty
AF5599
AF5599-SP
Detection of Mouse Ccd1/DIXDC1 by Western Blot.
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Mouse Ccd1/DIXDC1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ccd1/DIXDC1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Ccd1/DIXDC1
Gln481-Asn711
Accession # Q80Y83
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Ccd1/DIXDC1 antibody by Western Blot. View Larger

Detection of Mouse Ccd1/DIXDC1 by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Ccd1/DIXDC1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Ccd1/DIXDC1 in Mouse Embryo. Ccd1/DIXDC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ccd1/DIXDC1

Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.

Long Name
Coiled-coil DIX1/DIX Domain Containing 1
Entrez Gene IDs
85458 (Human); 330938 (Mouse); 363062 (Rat)
Alternate Names
Ccd1; Coiled-coil protein DIX1; Coiled-coil-DIX1; DIX domain containing 1; DIX domain-containing protein 1; Dixdc1; Dixin; Kiaa1735; KIAA1735coiled-coil-DIX1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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