Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ccd1/DIXDC1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Ccd1/DIXDC1
Gln481-Asn711
Accession # Q80Y83

Specificity

Detects mouse Ccd1/DIXDC1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Ccd1/DIXDC1 Antibody

Detection of Mouse Ccd1/DIXDC1 antibody by Western Blot.

Detection of Mouse Ccd1/DIXDC1 by Western Blot.

Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Ccd1/DIXDC1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Ccd1/DIXDC1 in Mouse Embryo.

Ccd1/DIXDC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Ccd1/DIXDC1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

1 µg/mL
Sample: Mouse brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ccd1/DIXDC1

Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.

Long Name

Coiled-coil DIX1/DIX Domain Containing 1

Alternate Names

Dixdc1, Dixin, Kiaa1735

Entrez Gene IDs

85458 (Human); 330938 (Mouse); 363062 (Rat)

Gene Symbol

DIXDC1

UniProt

Q80Y83 (Mouse)

Additional Ccd1/DIXDC1 Products

Product Documents for Mouse Ccd1/DIXDC1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Ccd1/DIXDC1 Antibody

For research use only

Citations for Mouse Ccd1/DIXDC1 Antibody

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Protocols

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