Mouse Ccd1/DIXDC1 Antibody Summary
Accession # Q80Y83
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Ccd1/DIXDC1 by Western Blot. Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Ccd1/DIXDC1 in Mouse Embryo. Ccd1/DIXDC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Coiled-coil-DIX1 (Ccd1), also known as DIXDC1, is an 80 kDa cytoplasmic actin-binding protein that mediates Wnt signaling by interacting with Dishevelled and Axin. It is highly expressed during nervous system development. Mouse Ccd1 contains a CH domain (aa 22‑152), a coiled-coil domain (aa 279‑452), and a DIX domain (aa 629‑705). Multiple alternately spliced isoforms of mouse Ccd11 are expressed in a tissue specific manner during development and in the adult. These variants may lack approximately one third of the N-terminal region of the molecule or carry short deletions within the CH domain or spacer region between the coiled-coil and DIX domains. Over aa 481‑711, mouse Ccd1 shares 88% and 75% aa sequence identity with human and rat Ccd1, respectively.
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