Mouse CCR10 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 248918
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C6 rat glioma cell line transfected with mouse CCR10
    Met1-Asn362
    Accession # NP_031747
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse CCR10 PE‑conjugated Monoclonal Antibody (Catalog # FAB2815P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR10 in BaF3 Mouse Cell Line Transfected with CCR10 and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with mouse CCR10 and eGFP was stained with Rat Anti-Mouse CCR10 PE‑conjugated Monoclonal Antibody (Catalog # FAB2815P) and. Quadrant markers were set based on control antibody staining (Catalog # IC013P). dView our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

  • Entrez Gene IDs:
    2826 (Human); 12777 (Mouse); 363682 (Rat)
  • Alternate Names:
    CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Adaptive immunity to murine skin commensals.
    Authors: Shen W, Li W, Hixon J, Bouladoux N, Belkaid Y, Dzutzev A, Durum S
    Proc Natl Acad Sci U S A, 2014;111(29):E2977-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009;182(11):6851-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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