Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 248918
View all CCR10 Primary Antibodies »

Product Specifications

Immunogen

C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747

Specificity

Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CCR10 Antibody

Detection of CCR10 antibody in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP antibody by Flow Cytometry.

Detection of CCR10 in HEK293 Human Cell Line Transfected with Mouse CCR10 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) mouse CCR10 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815) or Rat IgG2AIsotype Control (Catalog # MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR10 in Raw264.7 cells by Flow Cytometry.

Raw264.7 cells were stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR10 and eGFP; Raw264.7 mouse monocyte/macrophage cell line.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Alternate Names

CCR10, GPR2

Entrez Gene IDs

2826 (Human); 12777 (Mouse); 363682 (Rat)

Gene Symbol

CCR10

UniProt

NP_031747

Additional CCR10 Products

Product Documents for Mouse CCR10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse CCR10 Antibody

For research use only

Citations for Mouse CCR10 Antibody

Customer Reviews for Mouse CCR10 Antibody

There are currently no reviews for this product. Be the first to review Mouse CCR10 Antibody and earn rewards!

Have you used Mouse CCR10 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies