Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry.
B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR10 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.
Entrez Gene IDs:
2826 (Human); 12777 (Mouse); 363682 (Rat)
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2
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Cells were stained for 10 minutes in room temperature followed by three washes. Isotype control was done in parallel. Specificity: Reasonably specific Sensitivity: Not sensitive Buffer: PBS + 2.5% FBS Dilution: 1/10