Mouse/Rat CCR10 APC-conjugated Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 248918
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C6 rat glioma cell line transfected with mouse CCR10
    Met1-Asn362
    Accession # NP_031747
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR10 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

  • Entrez Gene IDs:
    2826 (Human); 12777 (Mouse); 363682 (Rat)
  • Alternate Names:
    CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential
    PLoS ONE, 2016;11(4):e0154253.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011;475(7355):226-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5.
    Authors: Uematsu S, Fujimoto K, Jang MH, Yang BG, Jung YJ, Nishiyama M, Sato S, Tsujimura T, Yamamoto M, Yokota Y, Kiyono H, Miyasaka M, Ishii KJ, Akira S
    Nat. Immunol., 2008;9(7):769-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse/Rat CCR10 APC‑conjugated Antibody FAB2815A
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Very Good
 Flow Mouse Anonymous 10/26/2015
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Flow Cytometry Mouse/Rat CCR10 APC‑conjugated Antibody FAB2815A
Flow Cytometry: Mouse/Rat CCR10 APC‑conjugated Antibody [FAB2815A]

Summary

ApplicationFlow Cytometry
Sample Testedactivated mouse CD8 T cell
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCells were stained for 10 minutes in room temperature followed by three washes. Isotype control was done in parallel.
Specificity: Reasonably specific
Sensitivity: Not sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10

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