Mouse CCR8 APC‑conjugated Antibody

R&D Systems | Catalog # FAB8324A

Recombinant Monoclonal Antibody.
R&D Systems
Discontinued Product
FAB8324A has been discontinued. View all CCR8 products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1055C
View all CCR8 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse CCR8
Accession # NP_031746

Specificity

Stains mouse CCR8 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse CCR8 APC‑conjugated Antibody

Detection of CCR8 antibody in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP antibody by Flow Cytometry.

Detection of CCR8 in HEK293 Human Cell Line Transfected with Mouse CCR8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP (top panel) or irrelevant transfectant (bottom panel) were stained with Rabbit Anti-Mouse CCR8 APC-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # FAB8324A). Quadrant markers were set based on control antibody staining (Catalog # IC105A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR8 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CCR8 and eGFP

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR8

CCR8 (C-C chemokine Receptor 8; also known as CD198) is a 41-43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O-glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1-33 and 92-105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Alternate Names

CCR8, CD198, CDw198, ChemR1, CKRL1, GPRCL1, TER-1

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Gene Symbol

CCR8

UniProt

NP_031746

Additional CCR8 Products

Product Documents for Mouse CCR8 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCR8 APC‑conjugated Antibody

For research use only

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