Mouse CD14 Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 159010
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD14
    Ala18-Pro345 (predicted)
    Accession # P10810
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD14 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD14 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB982N) and Rat Anti-Mouse F4/80/EMR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5580P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD14
CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
  • References:
    1. Wright, S.D. et al. (1990) Science 249:1431.
    2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
    3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
    4. Stelter, F. (2000) Chem. Immunol. 74:25.
  • Entrez Gene IDs:
    929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
  • Alternate Names:
    CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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