Mouse CD151 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4609T-100UG
Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry
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Mouse CD151 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 455807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse CD151
Met1-Tyr253
Accession # BAA22447
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry View Larger

Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB4609T, filled histogram) or isotype control antibody (Catalog # IC006T, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD151

CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94%-96% aa sequence identity with human and rat CD151.

Entrez Gene IDs
977 (Human); 12476 (Mouse); 64315 (Rat)
Alternate Names
CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151

Product Datasheets

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