Mouse CD23/Fc epsilon  RII Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6900T-025
Detection of CD23/Fc epsilon  RII in Mouse Splenocytes by Flow Cytometry.
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Mouse CD23/Fc epsilon  RII Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD23/Fc epsilon RII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc epsilon RIA or rhFc epsilon RIG is observed.
Source
Monoclonal Rat IgG2A Clone # 691632
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD23/Fc epsilon RII
Glu50-Pro331
Accession # P20693
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD23/Fc epsilon  RII in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD23/Fc epsilon  RII in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD23/Fc epsilon  RII Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB6900T) or (B) Rat IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC006T). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD23/Fc epsilon RII

The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2).  Coiled-coil topology within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa sequence identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease activity, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

References
  1. Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
  2. Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
  3. Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
  4. Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
  5. Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
  6. Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
  7. Sukumar, S. et al. (2006) J. Immunol. 176:4811.
  8. Karagiannis, S.N. et al. (2001) Immunology 103:319.
  9. Aubry, J-P. et al. (1992) Nature 358:505.
  10. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
  11. Montagnac, G. et al. (2005) Traffic 6:230.
  12. Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
  13. Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
  14. Entrez Accession # EDL21949, EDL21951.
  15. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.
Long Name
Fc epsilon Receptor II
Entrez Gene IDs
2208 (Human); 14128 (Mouse); 171075 (Rat)
Alternate Names
BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor

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