Mouse CD24 APC-conjugated Antibody

Recombinant Monoclonal Antibody

Discontinued Product

FAB8547A has been discontinued.
Detection of CD24 in Mouse Splenocytes by Flow Cytometry.
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Mouse CD24 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD24a in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 910019
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Peptide sequence spanning a portion of the extracellular region
Accession # P24807
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD24 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD24 APC‑conjugated Monoclonal Antibody (Catalog # FAB8547A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD24

CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.

Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

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