Mouse B220/CD45R PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1217P
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
1 Image
Product Details
Citations (2)
FAQs
Reviews

Mouse B220/CD45R PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

Product Datasheets

You must select a language.

x

Citations for Mouse B220/CD45R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Oral supplementation of diabetic mice with propolis restores the proliferation capacity and chemotaxis of B and T lymphocytes towards CCL21 and CXCL12 by modulating the lipid profile, the pro-inflammatory cytokine levels and oxidative stress.
    Authors: Al Ghamdi A, Badr G, Hozzein W, Allam A, Al-Waili N, Al-Wadaan M, Garraud O
    BMC Immunol, 2015-09-15;16(0):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. p53 Serves as a Host Antiviral Factor That Enhances Innate and Adaptive Immune Responses to Influenza A Virus
    Authors: César Muñoz-Fontela, Michael Pazos, Igotz Delgado, William Murk, Sathish Kumar Mungamuri, Sam W. Lee et al.
    The Journal of Immunology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse B220/CD45R PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse B220/CD45R PE-conjugated Antibody and earn rewards!

Have you used Mouse B220/CD45R PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review