Mouse CD30 Ligand/TNFSF8 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse CD30 Ligand/TNFSF8 in ELISAs and Western blots. In sandwich immunoassays, no cross‑reactivity or interference with recombinant human (rh) CD30, recombinant mouse (rm) CD30, rhCD40 Ligand, rhFas Ligand, rhTNF-alpha, rmTNF-alpha, recombinant rat TNF-alpha, or rhTNF-beta is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 82414
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD30 Ligand/TNFSF8
    Accession # P32972
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD30 Ligand/TNFSF8 in Mouse Splenocytes Activated with Concavalin A by Flow Cytometry. Mouse splenocytes activated with Concavalin A were stained with Rat Anti-Mouse CD30 Ligand/TNFSF8 APC‑conjugated Monoclonal Antibody (Catalog # FAB732A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD30 Ligand/TNFSF8

CD30 Ligand (CD30L)/TNFSF8 is a 39-40 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. CD30L is expressed on the cell surface of activated CD3+ CD4+ CD44+ T cells, and constitutively on mature CD11c+ CD40+ CD86++ CCR7+ CD plus thymic medullary epithelium, CD4+ CD3- CD11c- OX40L+ splenic accessory cells, and IL-17 secreting V gamma 6 gamma δ T cells. Although the protein has also been described on granulocytes, B cells and monocytes, its presence on these cells in mouse is uncertain. The specific receptor for CD30L is CD30/TNFRSF8, a 90-100 kDa type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. CD30 appears on virus-infected T and B cells and normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects on CD30L-expression cells, including a reduction in IL-2 production and the promotion of IL-17A secretion. CD30 may act as a co‑stimulatory molecule in thymic negative selection, and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas in human. Over aa 68-239, mouse CD30L shares 90% and 75% aa sequence identity with rat and human CD30L, respectively.

  • Entrez Gene IDs:
    944 (Human); 21949 (Mouse)
  • Alternate Names:
    CD153 antigen; CD153; CD30 antigen ligand; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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