Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CD63 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB5417R). Quadrant markers were set based on control antibody staining(Catalog # IC005R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.
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