|Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.|
|CD63 in Mouse Kidney. CD63 was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse CD63 Monoclonal Antibody (Catalog # MAB5417) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.|
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.
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