Mouse Cystatin/Stefin Homolog 2 Antibody

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Mouse Cystatin/Stefin Homolog 2 Antibody Summary

Species Reactivity
Detects mouse Cystatin/Stefin Homolog 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin D, S, SA, SN, recombinant mouse Cystain B, C, E/M, rhFetuin A, rhFetuin B, rhHPRG, or rhKininostatin is observed.
Monoclonal Rat IgG2B Clone # 237305
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Cystatin/Stefin Homolog 2
Accession # NP_084009
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Cystatin/Stefin Homolog 2
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cystatin H2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin/Stefin Homolog 2

RIKEN cDNA clone 2010005H15 (Nucleotide Accession # AK008122) predicted an intracellular protein, which was similar to members of the cystatin superfamily (1‑4). The protein is designated here as mouse Cystatin/Stefin Homolog 2 (Cystatin H2) because the deduced amino acid sequence showed the highest identity (75% over the entire length) to that of mouse cystatin/stefin homolog (SwissProt Accession # P56567).

  1. Carninci, P. and Hayashizaki, Y. (1999) Methods. Enzymol. 303:19.
  2. Carninci, P. et al. (2000) Genome Res. 10:1617.
  3. Shibata, K. et al. (2000) Genome Res. 10:1757.
  4. Kawai, J. et al. (2001) Nature 409:685.
Entrez Gene IDs
76770 (Mouse)
Alternate Names
010005H15Rik; Csta2; Cystatin H2; Cystatin/Stefin Homolog 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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