Detection of Mouse DMBT1 by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse DMBT1 Monoclonal Antibody (Catalog # MAB59151) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DMBT1 at approximately 210 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.
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