Mouse DMBT1 Antibody

Catalog # Availability Size / Price Qty
MAB59151
MAB59151-SP
Detection of Mouse DMBT1 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse DMBT1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DMBT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD163 or recombinant human SRCRB4D is observed.
Source
Monoclonal Rat IgG2B Clone # 548012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant mouse DMBT1
Trp215-Gly420
Accession # Q60997
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse DMBT1 by Western Blot. View Larger

Detection of Mouse DMBT1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse DMBT1 Monoclonal Antibody (Catalog # MAB59151) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DMBT1 at approximately 210 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMBT1

 

DMBT1 (deleted in malignant brain tumors 1), also called salivary agglutinin (SAG) in humans, hensin in rabbit, ebnerin in rat, and muclin, crp-ductin or vomeroglandin in mouse, is a transmembrane or secreted 2085 amino acid (aa) protein of the scavenger receptor cysteine-rich (SRCR) family. It contains several SRCR and CUB domains and a zona pellucida motif. The region used as the immunogen (aa 215-420) contains most of SRCR 2 and 3, and shares ~74% aa identity with corresponding regions of rat or human DMBT1. Several alternately spliced, glycosylated and sulfated forms exist. DMBT1 functions in mucosal immunity by binding bacterial, viral and endogenous proteins to aid clearance by macrophages. It is also thought to play a role in epithelial cell differentiation and tumor suppression.

Long Name
Deleted in Malignant Brain Tumors 1
Entrez Gene IDs
1755 (Human); 12945 (Mouse); 170568 (Rat)
Alternate Names
CRP-ductin; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; DMBT1; Ebnerin; FLJ61058; Glycoprotein 340; GP340; gp-340; GP340muclin; Hensin; MGC164738; Muclin; SAG; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein; Vomeroglandin

Product Datasheets

Citations for Mouse DMBT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model
    Authors: TB Tsay, MC Yang, WH Chang, PH Chen, LW Chen
    J Transl Med, 2018;16(1):225.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Toll-like receptor stimulation induces nondefensin protein expression and reverses antibiotic-induced gut defense impairment.
    Authors: Wu Y, Hsu C, Chen P, Fung C, Chen L
    Infect Immun, 2014;82(5):1994-2005.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse DMBT1 Antibody

There are currently no reviews for this product. Be the first to review Mouse DMBT1 Antibody and earn rewards!

Have you used Mouse DMBT1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review