Mouse Fas/TNFRSF6/CD95 APC-conjugated Antibody

FAB435A has been discontinued. View all Fas/TNFRSF6/CD95 products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Fas/TNFRSF6/CD95 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) Fas, rh4-1BB, rhDR6, rhNGF R, recombinant mouse (rm) OPG, rmRANK and rmCD30 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Fas/TNFRSF6/CD95
    Gly14-Arg169
    Accession # P25446
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Fas/TNFRSF6/CD95 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse Fas/TNFRSF6/CD95 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB435A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

  • References:
    1. Nagata, S. and P. Golstein (1995) Science 267:1449.
    2. Nagata, S. (1997) Cell 88:355.
    3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
    4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA. 94:5986.
  • Long Name:
    Fibroblast-associated
  • Entrez Gene IDs:
    355 (Human); 14102 (Mouse); 246097 (Rat); 493881 (Feline)
  • Alternate Names:
    Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6
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