Detects mouse Fas/TNFRSF6/CD95 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) Fas, rh4-1BB, rhDR6, rhNGF R, recombinant mouse (rm) OPG, rmRANK and rmCD30 is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Fas/TNFRSF6/CD95 Gly14-Arg169 Accession # P25446
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Fas/TNFRSF6/CD95 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Goat Anti-Mouse Fas/TNFRSF6/CD95 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB435A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.
Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.
Nagata, S. and P. Golstein (1995) Science 267:1449.
Nagata, S. (1997) Cell 88:355.
Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA. 94:5986.
Have you used Mouse Fas/TNFRSF6/CD95 APC-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.