Mouse Fc gamma RII/RIII (CD32/CD16) Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1460N-100UG
R&D Systems Antibodies
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse Fc gamma RII/RIII (CD32/CD16) Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse CD16 is observed and no cross-reactivity with recombinant human CD32 is observed.
Source
Monoclonal Rat IgG2b Clone # 190909
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr30-Arg207
Accession # P08101
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fc gamma RII/RIII (CD32/CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody‑dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8‑10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6‑10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine‑based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM‑bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine‑based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig‑like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16; Fc gamma RII/RIII

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Fc gamma RII/RIII (CD32/CD16) Alexa Fluor® 700-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse Fc gamma RII/RIII (CD32/CD16) Alexa Fluor® 700-conjugated Antibody and earn rewards!

Have you used Mouse Fc gamma RII/RIII (CD32/CD16) Alexa Fluor® 700-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review