Rat IgG2B Alexa Fluor® 700-conjugated Antibody

  
  • Specificity
    Selected for use as a rat IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2B Clone # 141945
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry Mouse splenocytes treated with PMA and Calcium Ionomycin for3 days were stained with Rat Anti-Mouse LAG-3Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog #FAB33281N,filled histogram) or Rat IgG Alexa Fluor® 700-conjugated IsotypeControl Antibody (Catalog # IC013N, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Rat IgG2B Fluorescein-conjugated Antibody

Ctrl IC013F 3  
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Rat IgG2B PerCP-conjugated Isotype Control

Ctrl IC013C
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Rat IgG2B Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC013V
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Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC013T
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Rat IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC013S
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Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC013R
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Rat IgG2B Biotinylated Isotype Control

Ctrl IC013B
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Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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