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OR

Mouse GITR/TNFRSF18 PE‑conjugated Antibody

1 citations
  • Specificity
    Detects mouse GITR/TNFRSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, recombinant mouse (rm) CD27, rmCD30, rmEDAR, rmFas, rhGITR, rhHVEM, rmRANK, rhTROY, and rmTNF R1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 108619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse GITR/TNFRSF18
    Met1-His153
    Accession # O35714
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GITR/TNFRSF18 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse GITR/TNFRSF18 PE-conjugated Monoclonal Antibody (Catalog # FAB5241P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glucocorticoid Induced TNF Receptor Family Related Gene

GITR (Glucocorticoid-Induced Tumor Necrosis Factor Receptor), also known as AITR, is a member of the co‑stimulatory subset of the TNF receptor superfamily (1, 2). In mouse, the GITR gene is composed of five exons and encodes multiple length isoforms that arise from alternate splicing. The “standard”, or first reported isoform is a type I transmembrane protein, 228 amino acids (aa) in length that contains a 19 aa signal sequence, a 134 aa extracellular region, a 21 aa transmembrane segment, and a 54 aa cytoplasmic domain. The extracellular region contains four potential N-linked glycosylation sites plus three cysteine-rich pseudorepeats of about 40 aa each (3, 4). The extracellular regions of mouse and human are 57% aa identical. The cytoplasmic domain has a P-x-Q/E-E motif that is known to associate with TRAF2. This is a common characteristic of TNFRSF members with co‑stimulatory functions (4). Three other mouse GITR isoforms (B, C and D) have been reported (5). All share the same N-terminal 101 of 134 aa in the extracellular region (including pseudorepeats #1, #2 and one-half of #3). Isoform D diverges at aa #101 and continues for another 12 aa for a total length of 113 aa. This is a naturally-occurring soluble form. Isoforms B and C show splicing in their cytoplasmic tails that creates cytoplasmic domains of 118 aa and 46 aa, respectively. In both the B and C isoforms, the TRAF2 binding site is spliced out, with a p56lck binding site inserted in isoform B (4). Given its membership in the TNFRSF, it likely functions as a trimer on the cell surface (2). GITR is predominantly expressed on CD4+CD25+ regulatory T cells (Treg) and naïve CD8+ and CD4+ CD25- T cells, where its expression is up-regulated after antigen-driven activation. GITR activation provides co‑stimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production (IL-2, IL-4, IFN-gamma ). On CD4+ CD25+ Treg cells, GITR activation provides co‑stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state (6‑8).

  • References:
    1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8. 
    2. Croft, M. (2003) Nat. Rev. Immunol. 3:609. 
    3. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
    4. Nocentini, G. et al. (2000) DNA Cell Biol. 19:205. 
    5. Nocentini, G. et al. (2000) Cell Death Differ. 7:408.
    6. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
    7. Ji, H. et al. (2004) J. Immunol. 172:5823.
    8. Stephens, G.L. et al. (2004) 173:5008.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat);
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; tumor necrosis factor receptor superfamily, member 18
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Applications
  1. Systemic anti-CD25 monoclonal antibody administration safely enhances immunity in murine glioma without eliminating regulatory T cells.
    Authors: Fecci PE, Sweeney AE, Grossi PM, Nair SK, Learn CA, Mitchell DA, Cui X, Cummings TJ, Bigner DD, Gilboa E, Sampson JH
    Clin. Cancer Res., 2006;12(14):4294-305.
    Species: Mouse
    Application: Flow Cytometry,

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  • Catalog #
    FAB5241P

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