Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # 1F1.93.2
View all IFN-gamma R1/CD119 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IFN‑ gamma R1/CD119
Extracellular domain

Specificity

Detects mouse IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IFN-gamma R1, rhIFN-gamma R2, or rhIL-10 R beta is observed.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Applications for Mouse IFN‑ gamma  R1/CD119 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IFN-gamma  R1/CD119 Fc Chimera (Catalog # 1026-GR)

Reviewed Applications

Read 1 review rated 5 using MAB10261 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN‑ gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN‑ gamma  R1 that binds IFN-gamma has been detected in biological fluids.

References

  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Gene Symbol

IFNGR1

Additional IFN-gamma R1/CD119 Products

Product Documents for Mouse IFN‑ gamma  R1/CD119 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN‑ gamma  R1/CD119 Antibody

For research use only

Citations for Mouse IFN‑ gamma  R1/CD119 Antibody

Customer Reviews for Mouse IFN‑ gamma  R1/CD119 Antibody (1)

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  • Mouse IFN-gamma  R1/CD119 Antibody
    Name: Klára Boháčová
    Application: Western Blot
    Sample Tested: Whole Cells
    Species: Mouse
    Verified Customer | Posted 01/18/2023
    50 ug of protein (KPC cell line), 500x diluted ab
    Mouse IFN‑ gamma  R1/CD119 Antibody MAB10261

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