Mouse IgG3 Fluorescein-conjugated Antibody

  
  • Specificity
    Selected for use as mouse IgG3 isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG3 Clone # 133316
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry NTera-2 human testicular embryonic carcinoma cell line wasstained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated MonoclonalAntibody (Catalog # FAB1435F,filled histogram) or Mouse IgG Fluorescein-conjugated Isotype Control Antibody(Catalog # IC007F, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G3
  • Alternate Names:
    DKFZp686H11213; FLJ39988; FLJ40036; FLJ40253; FLJ40587; FLJ40789; FLJ40834; IgG3; immunoglobulin heavy constant gamma 3 (G3m marker); MGC45809
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 Isotype Control

Ctrl MAB007 3  
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Mouse IgG3 APC-conjugated Antibody

Ctrl IC007A 1  
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Mouse IgG3 Alexa Fluor® 647-conjugated Antibody

Ctrl IC007R  
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Mouse IgG3 PE-conjugated Antibody

Ctrl IC007P
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