Mouse IgG3 Isotype Control

Catalog # Availability Size / Price Qty
MAB007
Detection of Mouse IgG Control by Flow Cytometry
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Product Details
Citations (5)
FAQs
Supplemental Products
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Mouse IgG3 Isotype Control Summary

Specificity
Selected for use as a mouse IgG3 isotype control. Recognizes KLH.
Source
Monoclonal Mouse IgG3 Clone # 133316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG3

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213; FLJ39988; FLJ40036; FLJ40253; FLJ40587; FLJ40789; FLJ40834; IgG3; immunoglobulin heavy constant gamma 3 (G3m marker); MGC45809

Product Datasheets

Citations for Mouse IgG3 Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. MUC20 expression marks the receptive phase of the human endometrium
    Authors: A Stepanjuk, M Koel, M Pook, M Saare, K Jääger, M Peters, K Krjutškov, S Ingerpuu, A Salumets
    Reprod. Biomed. Online, 2019;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats
    Authors: C Celik-Ozen
    Andrology, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.
    Authors: Thatava T, Kudva Y, Edukulla R, Squillace K, De Lamo J, Khan Y, Sakuma T, Ohmine S, Terzic A, Ikeda Y
    Mol Ther, 2013;21(1):228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  4. Resolvin E1 and chemokine-like receptor 1 mediate bone preservation.
    Authors: Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R
    J Immunol, 2013;190(2):689-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006;355(1):6-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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