Mouse IL-12 p70 Quantikine ELISA Kit

R&D Systems | Catalog # M1270

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL)

Sensitivity

2.5 pg/mL

Assay Range

7.8-500 pg/mL (Cell Culture Supernates, Serum)
View all IL-12 p70 ELISA Kits »

Product Summary for Mouse IL-12 p70 Quantikine ELISA Kit

The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse IL-12 p70

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 36 44 36
Mean (pg/mL) 28 68 193 20 56 230
Standard Deviation 2.5 6.5 19.4 2.3 6.2 20.0
CV% 8.9 9.6 10.1 11.5 11.1 8.7

Recovery for Mouse IL-12 p70 Quantikine ELISA Kit

The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 101 95-106
Serum (n=7) 97 88-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.

Mouse IL-12 p70 ELISA Linearity

Scientific Data Images for Mouse IL-12 p70 Quantikine ELISA Kit

Mouse IL-12 p70 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

IL-12 (Interleukin-12) is a secreted heterodimeric cytokine that contains disulfide-linked p35 and p40 subunits. The p40 subunit is also a subunit of the heterodimeric IL-23. IL-12 is produced by macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, microglia, and non-germinal center B cells. It acts  through a receptor complex that contains the ligand binding IL-12 R alpha 1 and the signal transducing IL-12 R alpha 2. IL-12 can enhance cytotoxic activity and induce Interferon-gamma production in NK cells, T cells and dendritic epidermal T cells. In conjunction with IL-23 and IL-27, IL-12 promotes the development of a Th1 immune response.

Long Name

Interleukin 12 p70

Alternate Names

IL12 p70

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Gene Symbol

IL12A

Additional IL-12 p70 Products

Product Documents for Mouse IL-12 p70 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-12 p70 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IL-12 p70 Quantikine ELISA Kit

Customer Reviews for Mouse IL-12 p70 Quantikine ELISA Kit (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse IL-12 p70 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Mouse IL-12 p70 Quantikine ELISA Kit
    Name: Fumou Sun
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 05/26/2023
    The Mouse IL-12 p70 ELISA Kit is stable. The R2 value of the standard curve is more than 0.99. Easy to use!
    Mouse IL-12 p70 Quantikine ELISA Kit M1270
  • Mouse IL-12 p70 Quantikine ELISA Kit
    Name: Mattie Baumann
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/26/2022
    Cell culture supernatants produced from transfected BHK-21 cells
    Mouse IL-12 p70 Quantikine ELISA Kit M1270
  • Mouse IL-12 p70 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/02/2018
  • Mouse IL-12 p70 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 10/07/2018
    Mouse IL-12 p70 Quantikine ELISA Kit M1270

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

View specific protocols for Mouse IL-12 p70 Quantikine ELISA Kit (M1270):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mouse IL-12 p70 Quantikine ELISA Kit

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for ELISA Kits