Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse IL‑17 Thr22-Ala158 Accession # Q62386
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑17/IL‑17A in Activated EL‑4 Mouse Cell Line by Flow Cytometry. Activated EL‑4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL‑17/IL‑17A Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+ Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium.
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