Mouse IL-18/IL-1F4 Antibody

Catalog # Availability Size / Price Qty
D048-3
Product Details
Citations (8)
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Mouse IL-18/IL-1F4 Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody reacts with mouse IL-18 on Immunoprecipitation.
Source
Monoclonal Rat IgG1 Clone # 93-10C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse IL-18/IL-1F4
Formulation
100 μg IgG in 100 μL volume of PBS containing 50% glycerol. No preservative is contained.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation
5 μg/0.5 μg recombinant mouse IL-18.
 
Neutralization
Induction of IFN- gamma by mouse IL-18 receptor transfected KG-1 cell (KG-1 cell: Human myelomonocyte: ATCC CCL246) in response to the 30 ng/mL recombinant mouse IL-18 was neutralized by this antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody solution is stable for one year from the date of purchase when stored at -20 °C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
  2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
  3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
  4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
  5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
  6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
  7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
  8. Okamura, H., et al., Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Mouse IL-18/IL-1F4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Hedgehog interacting protein–expressing lung fibroblasts suppress lymphocytic inflammation in mice
    Authors: Jeong H. Yun, ChangHee Lee, Tao Liu, Siqi Liu, Edy Y. Kim, Shuang Xu et al.
    JCI Insight
  2. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
    Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
    J Immunol, 2015-04-17;194(11):5407-16.
  3. Protein tyrosine phosphatase non-receptor type 22 modulates colitis in a microbiota-dependent manner
    Authors: MR Spalinger, TS Schmidt, M Schwarzfis, L Hering, K Atrott, S Lang, C Gottier, A Geirnaert, C Lacroix, X Dai, DJ Rawlings, AC Chan, C von Mering, G Rogler, M Scharl
    J. Clin. Invest., 2019-05-20;130(0):2527-2541.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Hypoxia induced mitogenic factor (HIMF) triggers angiogenesis by increasing interleukin-18 production in myoblasts
    Authors: CM Su, IC Wang, SC Liu, Y Sun, L Jin, SW Wang, HP Lee, WP Tseng, CH Tang
    Sci Rep, 2017-08-07;7(1):7393.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  5. Combination of the toll like receptor agonist and alpha-Galactosylceramide as an efficient adjuvant for cancer vaccine.
    Authors: Gableh F, Saeidi M, Hemati S, Hamdi K, Soleimanjahi H, Gorji A, Ghaemi A
    J Biomed Sci, 2016-01-25;23(1):16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. IL-18 regulates melanoma VLA-4 integrin activation through a Hierarchized sequence of inflammatory factors.
    Authors: Valcarcel M, Carrascal T, Crende O, Vidal-Vanaclocha F
    J Invest Dermatol, 2013-08-12;134(2):470-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation.
    Authors: Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ
    J. Biol. Chem., 2007-12-17;283(7):4200-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma.
    Authors: Kamath AT, Sheasby CE, Tough DF
    J. Immunol., 2005-01-15;174(2):767-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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