Mouse IL-19 Alexa Fluor® 647-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human
    IL-19 is observed.
  • Source
    Monoclonal Rat IgG2b Clone # 350105
  • Immunogen
    E. coli-derived recombinant mouse IL-19
    Leu25-Ala176
    Accession # Q8CJ70
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    Mouse whole blood cells fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2‑4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35‑45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

  • References:
    1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
    2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
    3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
    4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
    5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
    6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
    7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
    8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
    9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
    10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
    11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
    13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
    14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
    15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.
  • Long Name:
    Interleukin 19
  • Entrez Gene IDs:
    29949 (Human); 329244 (Mouse)
  • Alternate Names:
    IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
Related Research Areas

Have you used Mouse IL-19 Alexa Fluor® 647-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information