Mouse IL-19 Alexa Fluor® 647-conjugated Antibody

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IC29151R-100UG
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Mouse IL-19 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human
IL-19 is observed.
Source
Monoclonal Rat IgG2b Clone # 350105
Immunogen
E. coli-derived recombinant mouse IL-19
Leu25-Ala176
Accession # Q8CJ70
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Mouse whole blood cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2‑4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35‑45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

References
  1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
  4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
  5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
  6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
  7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
  8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
  13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.
Long Name
Interleukin 19
Entrez Gene IDs
29949 (Human); 329244 (Mouse)
Alternate Names
IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

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