Mouse IL-19 Antibody

Catalog # Availability Size / Price Qty
MAB29151
MAB29151-SP
Product Details
Citations (1)
FAQs
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Mouse IL-19 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human
IL-19 is observed.
Source
Monoclonal Rat IgG2B Clone # 350105
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-19
Leu25-Ala176
Accession # Q8CJ70
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑19 (Catalog # 2915-IL)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Mouse whole blood cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2‑4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35‑45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

References
  1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
  4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
  5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
  6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
  7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
  8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
  13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.
Long Name
Interleukin 19
Entrez Gene IDs
29949 (Human); 329244 (Mouse)
Alternate Names
IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19

Product Datasheets

Citation for Mouse IL-19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012;7(7):e40343.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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