Mouse IL-38/IL-1F10 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-38/IL-1F10 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 798036
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-38/IL-1F10
    Met1-Arg152
    Accession # Q8R459
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑38/IL‑1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑38/IL‑1F10 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7774G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-38/IL-1F10

IL-1F10, also known as IL-1HY2 and IL-38, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% amino acid identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

  • Long Name:
    Interleukin 1 Family Member 10
  • Entrez Gene IDs:
    84639 (Human); 215274 (Mouse)
  • Alternate Names:
    FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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