Mouse IL-3R beta APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5492A
Detection of IL‑3 R beta  in DA3 Mouse Myeloma Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse IL-3R beta APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑3 R beta in direct ELISAs. Cross-reactivity with AIC2B was not tested.
Source
Monoclonal Rat IgG2A Clone # 130705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-3 R beta  antibody in DA3 Mouse Myeloma Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑3 R beta in DA3 Mouse Myeloma Cell Line by Flow Cytometry. DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL-3 R beta APC-conjugated Monoclonal Antibody (Catalog # FAB5492A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

References
  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
Long Name
Interleukin 3 Receptor beta
Entrez Gene IDs
12984 (Mouse)
Alternate Names
AIC2A; BetaIl3; Bil3; Csfgmrb; IL3R beta; IL-3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse IL-3R beta APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-3R beta APC-conjugated Antibody and earn rewards!

Have you used Mouse IL-3R beta APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review