Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.
Monoclonal Rat IgG2B Clone # 334908
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 7 Phe34-Pro1036 Accession # NP_032424
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin alpha 7 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3518C, filled histogram) or isotype control antibody (Catalog # IC013C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 7
Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin beta 1 to bind Laminin 1.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.