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Mouse Integrin  alpha 7 Antibody

Catalog #: MAB3518
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3518
MAB3518-SP

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Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry.
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Citations (4)
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Mouse Integrin  alpha 7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.
Source
Monoclonal Rat IgG2B Clone # 334908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  alpha 7
Phe34-Pro1036
Accession # NP_032424
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a7 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Monoclonal Antibody (Catalog # MAB3518, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 7

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin  beta 1 to bind Laminin 1.

Entrez Gene IDs
3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)
Alternate Names
FLJ25220; integrin alpha 7 chain; Integrin alpha 7; integrin alpha-7; integrin, alpha 7; ITGA7

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Citations for Mouse Integrin  alpha 7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy(W) mouse model of MDC1A
    Authors: Packer D, Martin PT.
    Mol Ther Methods Clin Dev
  2. PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
    Authors: M Farnoodian, CM Sorenson, N Sheibani
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Super-Resolution Microscopy Reveals a Nanoscale Organization of Acetylcholine Receptors for Trans-Synaptic Alignment at Neuromuscular Synapses
    Authors: Amanda L. York, James Q. Zheng
    eNeuro
  4. Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma.
    Authors: Yu M, Eckart MR, Morgan AA, Mukai K, Butte AJ, Tsai M, Galli SJ
    J. Clin. Invest., 2011-08-01;121(8):3133-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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