Mouse IRF5 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8447R-100UG
Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry.
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Mouse IRF5 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IRF5 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 903430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IRF5
Ala208-Phe288
Accession # P56477
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry. View Larger

Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8447R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IRF5

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Long Name
Interferon Regulatory Factor 5
Entrez Gene IDs
3663 (Human); 27056 (Mouse); 296953 (Rat)
Alternate Names
IBD14; interferon regulatory factor 5; IRF5; IRF-5; SLEB10

Product Datasheets

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