Mouse IRF5 Antibody

Catalog # Availability Size / Price Qty
MAB8447
MAB8447-SP
Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry.
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Mouse IRF5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IRF5 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 903430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IRF5
Ala208-Phe288
Accession # P56477
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
A20 mouse B cell lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005); A20 mouse B cell lymphoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer kit (FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IRF5 antibody in A20 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005).View our protocol for Staining Intracellular Molecules.

Flow (Intracellular) View Larger

Detection of IRF5 in A20 cells by Flow Cytometry A20 cells were stained with Rat Anti-Mouse IRF5 Monoclonal Antibody (Catalog # MAB8447, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF5

IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.

Long Name
Interferon Regulatory Factor 5
Entrez Gene IDs
3663 (Human); 27056 (Mouse); 296953 (Rat)
Alternate Names
IBD14; interferon regulatory factor 5; IRF5; IRF-5; SLEB10

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