Mouse KLRG1 Antibody

Catalog # Availability Size / Price Qty
MAB69441-100
MAB69441-SP
Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry.
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Mouse KLRG1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse KLRG1 in direct ELISAs.
Source
Monoclonal Hamster IgG Clone # 2F1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
IL-2 activated NK cells from C57/BL6 mice
Accession # O88713
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Hamster Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB69441) or (B) Hamster IgG control antibody followed by APC-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLRG1

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus‑specific CD8+ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

References
  1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
  2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
  3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
  4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
  5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
  6. Voehringer, D. et al. (2002) Blood 100:3698.
  7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
  8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
  9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
  10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
  11. Thimme, R. et al. (2005) J. Virol. 79:12112.
  12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
  13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
  14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
  15. Banh, C. et al. (2009) Blood 114:5299.
Long Name
Killer Cell Lectin-like Receptor Subfamily G Member 1
Entrez Gene IDs
10219 (Human); 50928 (Mouse); 58975 (Rat)
Alternate Names
2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600

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