Mouse KLRG1 Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse KLRG1 in direct ELISAs.
  • Source
    Monoclonal Hamster IgG Clone # 2F1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    IL-2 activated NK cells from C57/BL6 mice
    Accession # O88713
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Hamster Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB69441) or (B) Hamster IgG control antibody followed by APC-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KLRG1

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus‑specific CD8+ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

  • References:
    1. Henson, S.M. and A.N. Akbar (2009) Age 31:285.
    2. Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
    3. Voehringer, D. et al. (2001) Immunogenetics 52:206.
    4. Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
    5. Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
    6. Voehringer, D. et al. (2002) Blood 100:3698.
    7. Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
    8. Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
    9. Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
    10. Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
    11. Thimme, R. et al. (2005) J. Virol. 79:12112.
    12. Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
    13. Ito, M. et al. (2006) J. Exp. Med. 203:289.
    14. Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
    15. Banh, C. et al. (2009) Blood 114:5299.
  • Long Name:
    Killer Cell Lectin-like Receptor Subfamily G Member 1
  • Entrez Gene IDs:
    10219 (Human); 50928 (Mouse); 58975 (Rat)
  • Alternate Names:
    2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600
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FAQs

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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse Anti-Hamster IgG Antibody

WB MAB011 2
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Mouse Anti-Hamster IgG Biotinylated Antibody

WB BAM011
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Mouse Anti-Hamster IgG PE-conjugated Antibody

Flow F0120
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Mouse Anti-Hamster IgG APC-conjugated Antibody

Flow F0121
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