Mouse LRRC32/GARP APC‑conjugated Antibody

R&D Systems | Catalog # FAB62291A

R&D Systems
Discontinued Product
FAB62291A has been discontinued. View all LRRC32/GARP products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 725226
View all LRRC32/GARP Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP
Ile18-Asn628
Accession # NP_001106850

Specificity

Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) LRRC32 is observed and no cross-reactivity with rhLRRC3, rhLRRC4, or rhNGL-3/LRRC4B is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse LRRC32/GARP APC‑conjugated Antibody

Detection of LRRC32/GARP antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stimulated with anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML) and TGF-beta for 3 days to induce regulatory T cell differentiation. Cells were stained with Rat Anti-Mouse CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rat Anti-Mouse LRRC32/GARP APC-conjugated Monoclonal Antibody (Catalog # FAB62291A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse LRRC32/GARP APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes stimulated with anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML) and TGF-beta

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LRRC32/GARP

Leucine-rich Repeat Protein 32 (LRRC32), also known as GARP (Glycoprotein A Repetitions Predominant), is an 77-80 kDa type I transmembrane glycoprotein. Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, followed by a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain. Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is expressed on hepatic stellate cells and on adult platelets. Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg). LRRC32 expression promotes the acquisition of a Treg phenotype that is characterized by reduced cellular proliferation and cytokine secretion, plus the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and, in association with alpha V beta 8 Integrin, mediates the activation and release of TGF-beta  from the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

Long Name

Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant

Alternate Names

D11S833E, GARP, Garpin

Entrez Gene IDs

2615 (Human); 434215 (Mouse)

Gene Symbol

LRRC32

UniProt

NP_001106850

Additional LRRC32/GARP Products

Product Documents for Mouse LRRC32/GARP APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse LRRC32/GARP APC‑conjugated Antibody

For research use only

Related Research Areas

Regulatory T Cells (Tregs)

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