Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB554F) or (B) Rat IgG2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
is a type I membrane glycoprotein belonging to the immunoglobulin
superfamily. It is expressed predominantly on thymocytes and a subset of
mature T lymphocytes. CD4 functions in collaboration with the T cell
receptor in the recognition of peptide antigens that are presented by
class II major histocompatibility complexes. CD4 also has been shown to
be a coreceptor of HIV entry and specifically binds gp120, the external
envelope glycoprotein of HIV.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.