Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Flow Cyomtetry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 226402
View all MDL-1/CLEC5A Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A

Specificity

Detects mouse MDL-1/CLEC5A. In direct ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse MDL‑1/CLEC5A PE‑conjugated Antibody

Detection of MDL-1/CLEC5A antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A PE-conjugated Monoclonal Antibody (Catalog # FAB1639P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MDL‑1/CLEC5A PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Long Name

Myeloid DAP12-associating Lectin

Alternate Names

CLEC5A, CLECSF5, MDL1

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Gene Symbol

CLEC5A

Additional MDL-1/CLEC5A Products

Product Documents for Mouse MDL‑1/CLEC5A PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MDL‑1/CLEC5A PE‑conjugated Antibody

For research use only

Citations for Mouse MDL‑1/CLEC5A PE‑conjugated Antibody

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