Mouse MDL-1/CLEC5A PE-conjugated Antibody

Catalog #: FAB1639P Datasheet / COA / SDS

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FAB1639P

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Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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Mouse MDL-1/CLEC5A PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MDL-1/CLEC5A. In direct ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 226402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MDL‑1/CLEC5A in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse MDL-1/CLEC5A PE-conjugated Monoclonal Antibody (Catalog # FAB1639P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Long Name

Myeloid DAP12-associating Lectin

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Alternate Names

CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1