Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

R&D Systems | Catalog # FAB6118A

R&D Systems
Discontinued Product
FAB6118A has been discontinued. An alternative/replacement product is available: NBP1-43312APC. View all MHC class II (I-A/I-E) products.

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # M5/114.15.2
View all MHC class II (I-A/I-E) Primary Antibodies »

Product Specifications

Immunogen

Activated C57BL/6 mouse spleen cells

Specificity

Detects mouse MHC class II (I‑A/I‑E) in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

Detection of MHC class II (I-A/I-E) antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F) and either (A) Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (Catalog # FAB6118A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MHC class II (I-A/I-E)

The M5/114.15.2 monoclonal antibody recognizes a homologous region of MHC class II associated I-A and I-E antigens (1). It binds a region shared by I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II antigens, which are expressed on antigen presenting cells (2).

References

  1. Bhattacharya, A. et al. (1981) J. Immunol. 127:2448.
  2. Watts, C. et al. (1997) Ann. Rev. Immunol. 15:821.

Long Name

Major Histocompatibility Complex Class II IA and IE

Alternate Names

H2-Ea, MHC class II IA/IE

Entrez Gene IDs

14968 (Mouse)

Gene Symbol

H2-EA-PS

Additional MHC class II (I-A/I-E) Products

Product Documents for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

For research use only

Citations for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

Customer Reviews for Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody and earn rewards!

Have you used Mouse MHC class II (I‑A/I‑E) APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies