Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) and either (A) Rat Anti-Mouse MHC class II (I‑A/I‑E) APC‑conjugated Monoclonal Antibody (Catalog # FAB6118A) or (B) Rat IgG2B Allophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: MHC class II (I-A/I-E)
The M5/114.15.2 monoclonal antibody recognizes a homologous region of MHC class II associated I-A and I-E antigens (1). It binds a region shared by I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II antigens, which are expressed on antigen presenting cells (2).
Bhattacharya, A. et al. (1981) J. Immunol. 127:2448.
Watts, C. et al. (1997) Ann. Rev. Immunol. 15:821.
Major Histocompatibility Complex Class II IA and IE
Have you used Mouse MHC class II (I-A/I-E) APC-conjugated Antibody?
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