Mouse CD3 Fluorescein-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) or (B) Rat IgG2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
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