Mouse MMR/CD206 APC-conjugated Antibody

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FAB25351A

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Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry.
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Citations (2)
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Mouse MMR/CD206 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MMR/CD206 in ELISAs.
Source
Monoclonal Rat IgG1 Clone # 857615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q61830
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MMR/CD206 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 APC-conjugated Monoclonal Antibody (Catalog # FAB25351A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewisa and ‑Lewisx (1, 7, 9). The FNII domain mediates Ca2+‑independent binding of collagens (2, 10). The CTLDs participate in Ca2+‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

References
  1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
  2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
  3. Harris, N. et al. (1992) Blood 80:2363.
  4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
  5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
  6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
  8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
  9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
  11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
  12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.
Long Name
Macrophage Mannose Receptor
Entrez Gene IDs
4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

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Citations for Mouse MMR/CD206 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period
    Authors: Wen-Heng Ji, Dan-Dan Li, Dan-Ping Wei, Ai-Qin Gu, Ying Yang, Jing-Pian Peng
    Frontiers in Immunology
  2. Histone deacetylase 9 deficiency exaggerates uterine M2 macrophage polarization
    Authors: Y Liu, M Du, HY Lin
    Journal of Cellular and Molecular Medicine, 2021-06-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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