Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 740404
View all OLFM-L3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OLFM-L3
Gln22-Val406
Accession # Q8BK62

Specificity

Detects mouse OLFM-L3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse OLFM‑L3 Antibody

Detection of Mouse OLFM-L3 antibody by Western Blot.

Detection of Mouse OLFM-L3 by Western Blot.

Western blot shows lysates of mouse skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse OLFM-L3 Monoclonal Antibody (Catalog # MAB7324) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for OLFM-L3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse OLFM‑L3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OLFM-L3

OLFM-L3, also called OLF44, is an approximately 50 kDa secreted scaffolding glycoprotein of the Olfactomedin/Noelin family. It is predominantly expressed in the placenta and early embryo, but at low levels in the adult. In Xenopus it influences dorsoventral patterning during early development by restricting dorsal Chordin activity. Mature mouse OLFM-L3 shares 95% and 97% aa identity with human and mouse OLFM-L3, respectively.

Long Name

Olfactomedin-Like 3

Alternate Names

HNOEL-iso, OLF44, OLFML3

Entrez Gene IDs

56944 (Human); 99543 (Mouse); 310743 (Rat)

Gene Symbol

OLFML3

UniProt

Q8BK62

Additional OLFM-L3 Products

Product Documents for Mouse OLFM‑L3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse OLFM‑L3 Antibody

For research use only

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