Mouse OLFM-L3 Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects human OLFM-L3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2B Clone # 740404
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human OLFM-L3
    Gln22-Val406
    Accession # Q8BK62
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Mouse OLFM-L3 by Western Blot. Western blot shows lysates of mouse skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse OLFM-L3 Monoclonal Antibody (Catalog # MAB7324) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for OLFM-L3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OLFM-L3
OLFM-L3, also called OLF44, is an approximately 50 kDa secreted scaffolding glycoprotein of the Olfactomedin/Noelin family. It is predominantly expressed in the placenta and early embryo, but at low levels in the adult. In Xenopus it influences dorsoventral patterning during early development by restricting dorsal Chordin activity. Mature mouse OLFM-L3 shares 95% and 97% aa identity with human and mouse OLFM-L3, respectively.
  • Long Name:
    Olfactomedin-Like 3
  • Entrez Gene IDs:
    56944 (Human); 99543 (Mouse); 310743 (Rat)
  • Alternate Names:
    HNOEL-iso; HNOEL-isoOLF44; hOLF44; OLF44; olfactomedin-like 3; olfactomedin-like protein 3; OLFML3; OLFM-L3

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 28  
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Normal Rat IgG Control

Ctrl 6-001-A 11
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB , Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 3  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL015 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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