Mouse OLFM-L3 Antibody

Catalog # Availability Size / Price Qty
MAB7324
MAB7324-SP
Detection of Mouse OLFM-L3 by Western Blot.
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Mouse OLFM-L3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse OLFM-L3 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 740404
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse OLFM-L3
Gln22-Val406
Accession # Q8BK62
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse OLFM-L3 antibody by Western Blot. View Larger

Detection of Mouse OLFM-L3 by Western Blot. Western blot shows lysates of mouse skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse OLFM-L3 Monoclonal Antibody (Catalog # MAB7324) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for OLFM-L3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OLFM-L3

OLFM-L3, also called OLF44, is an approximately 50 kDa secreted scaffolding glycoprotein of the Olfactomedin/Noelin family. It is predominantly expressed in the placenta and early embryo, but at low levels in the adult. In Xenopus it influences dorsoventral patterning during early development by restricting dorsal Chordin activity. Mature mouse OLFM-L3 shares 95% and 97% aa identity with human and mouse OLFM-L3, respectively.

Long Name
Olfactomedin-Like 3
Entrez Gene IDs
56944 (Human); 99543 (Mouse); 310743 (Rat)
Alternate Names
HNOEL-iso; HNOEL-isoOLF44; hOLF44; OLF44; olfactomedin-like 3; olfactomedin-like protein 3; OLFML3; OLFM-L3

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