Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.
Monoclonal Rat IgG2A Clone # 118125
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta Glu24-Leu738 Accession # O70458
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of OSM R beta in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse OSM R beta Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB662G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: OSM R beta
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM Receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.
Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
Have you used Mouse OSM R beta Alexa Fluor® 488-conjugated Antibody?
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