Mouse Protocadherin-12 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF7926AFP488

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Sheep IgG
View all Protocadherin-12 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Protocadherin-12 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PCDH-18 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse Protocadherin-12 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Protocadherin-12

PCDH12 (Protocadherin 12; also VE‑Cadherin 2) is a 150‑160 kDa glycoprotein member of the PCDH family of molecules. It exhibits limited expression, being found on endothelial cells, renal mesangium, Sertoli cells, and glycogen‑positive trophoblast cells. PCDH is a transmembrane protein that forms homotypic aggregates in a calcium‑dependent manner. Although this would suggest a prominent role in cell-cell adhesion, it is unclear if this is the only activity for the molecule. In any case, it appears that PCDH12 does play an important role in placental development. Mature mouse PCDH12 is an 1163 amino acid (aa) type I transmembrane protein (aa 18‑1180). It possesses a 699 aa extracellular region (aa 18‑716) that contains six cadherin domains (aa 28‑711), plus a 21 aa transmembrane segment coupled to a lengthy 443 aa cytoplasmic domain (aa 738‑1180). Based on human, mouse PCDH12 will undergo proteolytic processing. MMP activity will first generate a 90 kDa circulating extracellular fragment and 60 kDa membrane‑bound fragment, followed by gamma ‑secretase which has the potential to further act on the membrane‑bound fragment, generating a cytosolic 50 kDa fragment. There is one potential alternative start site at Met1060. Over aa 18‑716, mouse PCDH12 shares 95% and 82% aa sequence identity with rat and human PCDH12, respectively.

Alternate Names

PCDH12, Protocadherin12, VE-cad-2, VE-cadherin-2

Entrez Gene IDs

51294 (Human); 53601 (Mouse)

Gene Symbol

PCDH12

UniProt

O55134 (Mouse)

Additional Protocadherin-12 Products

Product Documents for Mouse Protocadherin-12 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Protocadherin-12 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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