Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

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Detection of GABAAR  alpha 1 N-Terminus by Western Blot
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Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody Summary

Species Reactivity
Mouse, Rat, Bovine
Mouse and rat ~51 kDa GABAA R alpha 1 subunit, N-Terminus
Polyclonal Rabbit IgG
Antigen Affinity-purified
N-terminus peptide of GABAA R alpha 1 subunit conjugated to keyhole limpet hemocyanin (KLH)
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of GABA<sub>A</sub>R a1 N-Terminus antibody by Western Blot View Larger

Detection of GABAAR alpha 1 N-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAAR, alpha 1 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~51 kDa alpha 1 subunit of the GABAAR (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 1

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
  4. Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
  5. Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide
Entrez Gene IDs
2554 (Human); 14394 (Mouse); 29705 (Rat); 282235 (Bovine)
Alternate Names
ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABA A R alpha 1; GABAAR alpha 1; GABAARa1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1

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