Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

R&D Systems | Catalog # PPS023

R&D Systems
Discontinued Product
PPS023 has been discontinued. View all GABA-A R alpha 1 products.

Key Product Details

Species Reactivity

Mouse, Rat, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GABA-A R alpha 1 Primary Antibodies »

Product Specifications

Immunogen

N-terminus peptide of GABAA R alpha 1 subunit conjugated to keyhole limpet hemocyanin (KLH)

Specificity

Mouse and rat ~51 kDa GABAA R alpha 1 subunit, N-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

Detection of GABAAR a1 N-Terminus antibody by Western Blot

Detection of GABAAR alpha 1 N-Terminus by Western Blot

Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAAR, alpha 1 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~51 kDa alpha 1 subunit of the GABAAR (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.

Applications for Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Mouse brain (forebrain) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 1

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
  4. Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
  5. Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide

Alternate Names

EJM, GABAAR alpha 1, GABAARa1, GABRA1

Entrez Gene IDs

2554 (Human); 14394 (Mouse); 29705 (Rat); 282235 (Bovine)

Gene Symbol

GABRA1

Additional GABA-A R alpha 1 Products

Product Documents for Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

Certificate of Analysis

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Product Specific Notices for Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody

For research use only

Related Research Areas

Cancer Biomarkers
GABA Receptors

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