Mouse/Rat/Bovine GABA-A R alpha 1 N-Terminus Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of GABAAR alpha 1 N-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAAR, alpha 1 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~51 kDa alpha 1 subunit of the GABAAR (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.
Preparation and Storage
Background: GABA-A R alpha 1
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.
- Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
- Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
- Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
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