Home / Caspase-12 / Mouse/Rat Caspase-12 Antibody

Mouse/Rat Caspase-12 Antibody

Catalog #: AF1456
2 Citations Datasheet / COA / SDS

Discontinued Product

AF1456 has been discontinued.
View all Caspase-12 products.
Detection of Mouse/Rat Caspase‑12 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Caspase-12 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects endogenous mouse and rat Casapase-12 proenzyme in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Caspase-12
Met1-Asn419 (predicted)
Accession # O08736
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat Caspase‑12 antibody by Western Blot. View Larger

Detection of Mouse/Rat Caspase‑12 by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse/Rat Caspase-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1456) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Caspase-12 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-12

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Entrez Gene IDs
100506742 (Human); 12364 (Mouse); 156117 (Rat)
Alternate Names
Apoptosis Related Cysteine Protease; CASP 12; CASP12; CASP12P1; Caspase 12 pseudogene 1; Caspase12; Caspase-12; UNQ9415

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse/Rat Caspase-12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Inhibitory effect ofquercetinon titaniumparticle-inducedendoplasmic reticulum stress (ERS)-related apoptosisandin vivoosteolysis
    Authors: L Zhang, Z Tian, W Li, X Wang, Z Man, S Sun
    Biosci. Rep., 2017-07-31;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.
    Authors: Wang P, Arjona A, Zhang Y
    Nat. Immunol., 2010-09-05;11(10):912-9.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse/Rat Caspase-12 Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat Caspase-12 Antibody and earn rewards!

Have you used Mouse/Rat Caspase-12 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review