hh Mouse/Rat CCR9 APC-conjugated Antibody FAB2160A-025: R&D Systems

Mouse/Rat CCR9 APC-conjugated Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 242503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse CCR9
    Met1-Leu369
    Accession # Q9WUT7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR9 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR9 APC‑conjugated Monoclonal Antibody (Catalog # FAB2160A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse/Rat CCR9 APC‑conjugated Monoclonal Antibody (Catalog # FAB2160A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain chemokine receptor that serves as a receptor for CCL25/TECK. CCR9 is expressed on mature and immature thymocytes and some peripheral T and B cells.

  • Entrez Gene IDs:
    10803 (Human); 12769 (Mouse); 282832 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses.
    Authors: Gohda M, Kunisawa J, Miura F, Kagiyama Y, Kurashima Y, Higuchi M, Ishikawa I, Ogahara I, Kiyono H
    J. Immunol., 2008;180(8):5335-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5.
    Authors: Uematsu S, Fujimoto K, Jang MH, Yang BG, Jung YJ, Nishiyama M, Sato S, Tsujimura T, Yamamoto M, Yokota Y, Kiyono H, Miyasaka M, Ishii KJ, Akira S
    Nat. Immunol., 2008;9(7):769-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse/Rat CCR9 APC‑conjugated Antibody FAB2160A
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 Flow Mouse Anonymous 10/26/2015
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Flow Cytometry Mouse/Rat CCR9 APC‑conjugated Antibody FAB2160A
Flow Cytometry: Mouse/Rat CCR9 APC‑conjugated Antibody [FAB2160A]

Summary

ApplicationFlow Cytometry
Sample Testedactivated mouse CD8 T cell
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCells were stained for 10 minutes in room temperature followed by 3 washes.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS + 10% FBS
Dilution: 1/10

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