MIF Antibody (811429)

R&D Systems | Catalog # MAB1978

R&D Systems
Discontinued Product
MAB1978 has been discontinued. View all MIF products.

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 811429
View all MIF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse MIF
Met1-Ala115
Accession # P34884

Specificity

Detects mouse MIF in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MIF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for MIF Antibody (811429)

Detection of Mouse MIF antibody by Western Blot.

Detection of Mouse MIF by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB1978) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat MIF antibody by Western Blot.

Detection of Rat MIF by Western Blot.

Western blot shows lysates of NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB1978) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for MIF Antibody (811429)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIF

MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13 kDa member of the MIF family of proteins. Although secreted, it possesses no definitive signal sequence. MIF is widely expressed and serves to regulate immune reactions. On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling. On monocytes, this promotes TNF alpha  production plus IFN-gamma -stimulated NO production. On B cells, this promotes B cell survival. MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration. A tautomerase activity has also been shown for MIF and is suggested to involve the entire length of the molecule. Mouse MIF is 114 amino acids (aa) in length. MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60. MIF is considered to act as a homotrimer. Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively.

Long Name

Macrophage Migration Inhibitory Factor

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Entrez Gene IDs

4282 (Human); 17319 (Mouse)

Gene Symbol

MIF

UniProt

P34884

Additional MIF Products

Product Documents for MIF Antibody (811429)

Certificate of Analysis

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Product Specific Notices for MIF Antibody (811429)

For research use only

Citations for MIF Antibody (811429)

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