Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 274914
View all ROBO4 Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with mouse ROBO4
Leu28-Ser1012
Accession # Q8C310

Specificity

Detects mouse ROBO4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse ROBO4 Antibody

Detection of ROBO4 antibody in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP antibody by Flow Cytometry.

Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry.

Jurkat human acute T cell leukemia cell line transfected with (A) mouse ROBO4 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse ROBO4 Monoclonal Antibody (Catalog # MAB50041) followed by APC-conjugated Goat anti-Rat IgG secondary antibody Catalog # F0113. Quadrant markers were set based on Rat IgG2A Isotype Control (Catalog # MAB006, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ROBO4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROBO4

Roundabout receptors are molecular guidance proteins that function by interaction with Slit proteins to regulate axon guidance, neuronal migration, and leukocyte chemotaxis. ROBO4 expression is restricted to the endothelium and may also play a role in angiogenesis and endothelial cell migration.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8C310

Additional ROBO4 Products

Product Documents for Mouse ROBO4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ROBO4 Antibody

For research use only

Citations for Mouse ROBO4 Antibody

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