Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry.
Jurkat human acute T cell leukemia cell line transfected with (A) mouse ROBO4 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse ROBO4 Monoclonal Antibody (Catalog # MAB50041) followed by APC-conjugated Goat anti-Rat IgG secondary antibody Catalog # F0113. Quadrant markers were set based on Rat IgG2A Isotype Control (Catalog # MAB006, data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Roundabout receptors are molecular guidance proteins that function by interaction with Slit proteins to regulate axon guidance, neuronal migration, and leukocyte chemotaxis. ROBO4 expression is restricted to the endothelium and may also play a role in angiogenesis and endothelial cell migration.
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