Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 757129
View all Semaphorin 4A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A
Thr33-Trp638
Accession # Q62178

Specificity

Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untransfected cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Semaphorin 4A APC‑conjugated Antibody

Detection of Semaphorin 4A antibody in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP antibody by Flow Cytometry.

Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A APC-conjugated Monoclonal Antibody (Catalog # FAB8166A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Semaphorin 4A APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.

References

  1. Delgoffe, G.M. et al (2013) Nature 501:252.

Alternate Names

CORD10, SEMA4A, SEMAB, SEMB

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Gene Symbol

SEMA4A

UniProt

Q62178

Additional Semaphorin 4A Products

Product Documents for Mouse Semaphorin 4A APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Semaphorin 4A APC‑conjugated Antibody

For research use only

Related Research Areas

Proteoglycans

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