Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A APC‑conjugated Monoclonal Antibody (Catalog # FAB8166A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Semaphorin 4A
Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.
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